Enzyme Lab

Words: 1417
Pages: 6
Open Document
Introduction:
Enzymes are a type of protein that initiate and assist every chemical reaction that occurs. Enzymes have the ability to do this with help from a few things. Increasing the temperature helps increase reaction rates however if you go past the optimum temperature it denaturizes the enzyme and the reaction rate decreases. PH can also have an effect on enzymes go past the optimum pH and it can disrupt the enzyme function in the interactions between excess hydrogen or hydroxide ions and amino acid side chains which also causes the reaction rate to decrease. An increase in substrate can increase the amount of enzymes which in turn can increase the reaction rate. This can also be seen if you increase the amount of enzymes the amount …show more content…
A piece of tape has ‘tube #4’ written on it, it was then placed on an empty tube. The liquids were converted from ml to μl. Guaiacol was converted from 0.1 ml to 100 μl and was extracted using a 200 μl pipette that was adjusted to 100 μl. Guaiacol was then placed in tube #4. The tip from the 200 μl was disposed of and a new one was placed on it. H202 was converted from 0.2 ml to 200 μl which was also extracted by the 200 μl pipette. H202 was also placed in tube #4. The tip from the 200 μl was disposed of and a new one was placed on it. DH20 was not converted from its 4.7 ml. DH20 was extracted by the 5 ml pipette and then placed in tube #4. Another piece of tape has ‘tube #5’ written on it, it was then placed on an empty tube. Turnip extract was converted from 1.0 ml to 1000 μl. Turnip extract was extracted by the 1000 μl pipette and was then placed in tube #5. The tip on the 1000 μl was disposed of and a new was placed on it. DH20 wasn’t converted from 4.0 ml and was extracted by the 5.0 ml pipette. DH20 was then placed in tube #5. Tube #4 and tube #5 are both placed in water that is heated at 72°c for five minutes. Each person in each group was assigned as a mixer, a reader, a timer and a data recorder. A timer was started and tube #4 was poured into tube #5 then this solution was poured into a cuvette to the line on it and then placed in the spectrophotometer and the first recording for this experiment is taken when the timer hit 20 seconds. For the next 10 minutes every 20 seconds the absorbance is recorded in table
Show More

Related Documents: Enzyme Lab

Enzyme Lab Report

of reactivity of an enzyme, and what the optimal pH for the enzyme is. The answer to these questions are yes, pH affects the reactivity rate of an enzyme, and the optimal pH for the enzyme is about 9. Answering the questions, pH greatly affects the rate of reactivity of an enzyme. This is evident in figure 2 above. Looking at the trend of the data, when the pH was either too basic, or too acidic, the rate of reactivity was very low. This happens because of denaturation. Enzymes have an optimal pH…

Words 621 - Pages 3

Lab Enzyme Essay

Observation: Enzymes are proteins that help speed up chemical reactions and as such, are subject to change when heat, salt or pH are involved. Enzymes have evolved to withstand such factors in which they reside so, if there are different factors like pH and heat involved, the effect of a specific enzyme may not work as well or even better in different conditions compared to other types of enzymes. Another type of factor as well is what kind of organism uses the enzyme and if they have any conditions…

Words 837 - Pages 4

Potato Enzyme Lab

A Study on Enzyme Kinetics by Substrate Concentration and pH Colin Kuehnel Abstract An enzyme is capable of increasing rate (speed) of a reaction by lowering the activation energy of said reaction, specifically in our case the enzyme catalase. Like most things in the world, enzymes are subject to their environments and have ideal conditions in which they perform the best. Our experiment tested the performance of catalase under a variety of conditions including fluctuating pH, presence of inhibitors…

Words 1890 - Pages 8

Enzyme Lab Report

Enzyme Lab Report Enzymes are proteins that help speed up chemical reactions in your body. They help with our digestion and our metabolism. When enzymes speed up our chemical reactions its called catalysis. In order for the enzymes to catalysis it needs help from a molecule called a substrate. The substrate then binds to an active site. Every active site has it’s own little molecule or molecule’s that fit it. Lactase is an enzyme that is found in the small intestine. It breaks down lactose (milk…

Words 1567 - Pages 7

Enzyme Lab Report

Enzyme Action: Testing Catalase Activity Introduction Many organisms can decompose hydrogen peroxide enzymatically. Enzymes are proteins that are responsible for most of the chemical activities of living organisms. Enzymes act as catalysts, or substances that speed up the rate of chemical reactions. Enzyme activity is affected greatly under various conditions, such as different enzyme concentrations, pH values, and temperatures. Purpose To determine if differences in enzyme concentration, pH values…

Words 1986 - Pages 8

Enzyme Lab Report

By completing the enzyme lab it became evident that temperature directly correlates with enzyme activity. The chilled water and hydrogen peroxide created the least amount of enzyme activity, the control the second most, and lastly the hot water and hydrogen peroxide had the most activity. The class data proves this by showing that when catalase and hot hydrogen peroxide were placed in hot water, 62 millimeters of oxygen were produced. The cold water and hydrogen peroxide only produced 28 millimeters…

Words 408 - Pages 2

Enzyme Lab Essay

LABORATORY REPORT (Click on the Save a Copy button on the panel above to save your report) Activity: Enzyme Activity Predictions 1. Sucrase will have the greatest activity at pH 6 2. Sucrase will have the greatest activity at 60 °C (140 °F) 3. Sucrase activity decreases with increasing sucrose concentration. Materials and Methods Effect of pH on Enzyme Activity. 1. Dependent Variable. amount of product (glucose and fructose) produced 2. Independent Variable. pH 3. Controlled Variables…

Words 1196 - Pages 5

Enzyme Lab Report

accelerate chemical reactions is known as an enzyme. Enzymes contain a specific site where the reaction will take place, known as the active site. This site is unique where only a certain type of molecule, substrate, may attach. The reaction will take place and the products of the reaction will be released. There are two main hypotheses on the shape of an enzyme. The first one is the lock and key hypothesis. This hypothesis explains that the shape of the enzyme and the shape of the substrate are so specific…

Words 319 - Pages 2

Lactase Enzyme Lab Report

Lactase Enzyme Lab This lab will examine the specificity of an enzyme (lactase) to a specific substrate (lactose). You and your lab partner will observe the actions of the enzyme and how shape is important to enzyme reactions. Lactose, the sugar found in milk, is a disaccharide composed of glucose and galactose (both six-sided sugars). Sucrose, ordinary table sugar, is also a disaccharide composed of fructose and glucose. Glucose is a six-sided sugar and fructose is a five-sided sugar. Lactase…

Words 569 - Pages 3

Enzyme Peroxidase Lab Report

Enzyme is a substance produced by a living organism that acts as a catalyst to bring about a specific biochemical reaction. The purpose of this lab is to perform a quantitative essay of the activity of an enzyme in a tissue extract using a spectrophotometer. The second part of the lab is to then organize the data as concise tables and graphs for inclusion in a lab report describing the properties of the enzyme peroxidase. The third objective of the lab is to test the following hypotheses. The…

Words 522 - Pages 3