Enzyme Lab Report

Enzyme Lab Report
Enzymes are proteins that help speed up chemical reactions in your body. They help with our digestion and our metabolism. When enzymes speed up our chemical reactions its called catalysis. In order for the enzymes to catalysis it needs help from a molecule called a substrate. The substrate then binds to an active site. Every active site has it’s own little molecule or molecule’s that fit it.
Lactase is an enzyme that is found in the small intestine. It breaks down lactose (milk sugars) into simple sugar’s (glucose and galactose). There is a medical conditions known as being lactose and tolerant. That means that your stomach doesn’t break down the lactose. The withdrawals of being lactose and tolerant are cramps, production …show more content…
Label A as 4ºC, B as 25º C, C as 100ºC , D as pH 4, E as pH 7 and F as pH 10 . Then add 2 ml of milk to test tubes A-F. On test tubes A-C ( test tube A put in an ice bucket for 4ºC, test tube B put on lab bench for 25ºC, and test tube C put in 100º C water bath for 100ºC) put them in the environment of the corresponding temperature labeled. Leave test tubes A-C alone, now set a timer for two minutes and set aside. Quickly add 1 ml of lactase to test tubes D-F, now start the timer. At the end of the two minutes place a glucose tests strip in each tube and remove it then record it on table 3. Now return to the temperature experiment and set timer for ten minutes and set it aside. Quickly add 1 ml of lactase to test tube A-C and leave it in the temperature corresponding to each test tube and start the timer. In the end of the 10 minute time test for glucose by placing one glucose test strip in each tube and remove it, then record this date in table 2. Sections 3: Results: Experiment 1
The records shows that in figure 1a and figure 1b,treatment A – D have milk and sucrose as the control variable. The experiment group for treatment A-D is the lactase and the placebo is the water. In treatment A (milk + lactase) the glucose results were moderate glucose 100 ml/dl, treatment B (milk + water) the glucose results were no glucose 0 ml/dl, treatment C (sucrose + lactase) the glucose results were no glucose 0 ml/dl. Treatment D (sucrose + water) the glucose results …show more content…
The experimental group for treatment A-C is the different temperatures. Treatment A (milk + lactase) have temperature of 4ºC and the glucose results were 100 mg/dL. Treatment B (milk + lactase) has temperature of 25ºC and the glucose results were 100 mg/dL. Treatment C (milk + lactase) has temperature 100º C and glucose results were 0 mg/dL.
Treatments Glucose Results
A. Milk + Lactase, 4ºC 100 mg/dL
B. Milk + Lactase, 25ºC 100 mg/ dL
C. Milk + Lactase, 100ºC 0 mg/dL
Figure 2A

Results: Experiment 2B
The results show that in figure 2B and 2b, treatments D-F have milk and lactase as the control variable. The experimental group is the pH levels in each test tube. Treatment D ( milk + lactase) had pH level 4 and had glucose results 500 mg/dL. Treatment E (milk + lactase) has pH level 7 and had glucose results 250 mg/dL. Treatment F (milk + lactase) has pH level 10 and glucose results 0 mg/dL.

Treatment Glucose Results
D. Milk + Lactase, pH 4 500 mg/dL
E. Milk + Lactase, pH 7 250 mg/dL
F. Milk + Lactase, pH 10 0 mg/dL

Figure 2b

Section 4: Discussion and

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