Enzyme Lab Report Enzymes are proteins that help speed up chemical reactions in your body. They help with our digestion and our metabolism. When enzymes speed up our chemical reactions its called catalysis. In order for the enzymes to catalysis it needs help from a molecule called a substrate. The substrate then binds to an active site. Every active site has it’s own little molecule or molecule’s that fit it. Lactase is an enzyme that is found in the small intestine. It breaks down lactose (milk…
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of reactivity of an enzyme, and what the optimal pH for the enzyme is. The answer to these questions are yes, pH affects the reactivity rate of an enzyme, and the optimal pH for the enzyme is about 9. Answering the questions, pH greatly affects the rate of reactivity of an enzyme. This is evident in figure 2 above. Looking at the trend of the data, when the pH was either too basic, or too acidic, the rate of reactivity was very low. This happens because of denaturation. Enzymes have an optimal pH…
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accelerate chemical reactions is known as an enzyme. Enzymes contain a specific site where the reaction will take place, known as the active site. This site is unique where only a certain type of molecule, substrate, may attach. The reaction will take place and the products of the reaction will be released. There are two main hypotheses on the shape of an enzyme. The first one is the lock and key hypothesis. This hypothesis explains that the shape of the enzyme and the shape of the substrate are so specific…
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Enzyme is a substance produced by a living organism that acts as a catalyst to bring about a specific biochemical reaction. The purpose of this lab is to perform a quantitative essay of the activity of an enzyme in a tissue extract using a spectrophotometer. The second part of the lab is to then organize the data as concise tables and graphs for inclusion in a lab report describing the properties of the enzyme peroxidase. The third objective of the lab is to test the following hypotheses. The…
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By completing the enzyme lab it became evident that temperature directly correlates with enzyme activity. The chilled water and hydrogen peroxide created the least amount of enzyme activity, the control the second most, and lastly the hot water and hydrogen peroxide had the most activity. The class data proves this by showing that when catalase and hot hydrogen peroxide were placed in hot water, 62 millimeters of oxygen were produced. The cold water and hydrogen peroxide only produced 28 millimeters…
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Enzyme Action: Testing Catalase Activity Introduction Many organisms can decompose hydrogen peroxide enzymatically. Enzymes are proteins that are responsible for most of the chemical activities of living organisms. Enzymes act as catalysts, or substances that speed up the rate of chemical reactions. Enzyme activity is affected greatly under various conditions, such as different enzyme concentrations, pH values, and temperatures. Purpose To determine if differences in enzyme concentration, pH values…
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INTRODUCTION Enzymes are biological catalyst that alters the chemical reaction rate without itself being altered which reacts with the substrate and converts the enzyme substrate complex into different molecules – product. Enzyme plays the consequential role in functioning of life process such as for growth, digestion of nutrients, excretion of metabolic waste, energy provider to brain and muscles and thus directly or indirectly involved in every biological processing of life. Apart from numerous…
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Introductory Biology 1 Biology 1003 Fall Term 2011 Lab Number: 3 Title: Cell Energetics: Enzyme Role in Biological Reactions Name: Brandon Moore Student Number: 100819124 Lab day and time: Wednesday pm Date: Wednesday November 23, 2011 Introduction Enzymes are a key aspect in our everyday life and are a key to sustaining life. They are biological catalysts that help speed up the rate of reactions. They do this by lowering the activation energy of chemical reactions (Biology…
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The effect of low pH on Enzyme Activity Introduction What are Enzymes? Enzymes are proteins that control the speed of reaction in our body. They help in speeding up metabolic reaction in our bodies. If enzymes were absent, the speed of these metabolic reactions would be so slow that they would not be able to produce the required energy to keep us alive. Other functions of enzymes are to help cells to communicate with each other, keeping cell growth, life and death under control. On the other hand…
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Lactase Enzyme Lab This lab will examine the specificity of an enzyme (lactase) to a specific substrate (lactose). You and your lab partner will observe the actions of the enzyme and how shape is important to enzyme reactions. Lactose, the sugar found in milk, is a disaccharide composed of glucose and galactose (both six-sided sugars). Sucrose, ordinary table sugar, is also a disaccharide composed of fructose and glucose. Glucose is a six-sided sugar and fructose is a five-sided sugar. Lactase…
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