Nt1310 Unit 1 Lab Report

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1. Parameters that define substrate characteristics:
Substrate characteristics parameters basically include the attributes that define the substrate, such as the elasticity of the substrate used, Poisson's ration of the substrate, thickness of the substrate, diameter of the fluorescent beads embedded into the substrates, etc.
a. TITLE: This is the first line of the template file. The user should edit the existing line with a descriptive title that best defines their experiment. For this particular experiment, the title could include cell type, substrate type, the stiffness of the substrate used, and macrophage phenotype.
b. YMODU: In this parameter line, the user specifies Young's modulus (elasticity) of the substrate used. The value of YMODU should be entered in N/m2 (Pascals). For PAA gel of 2.3 kPa, the format of YMODU looks like 2.300E+03. The template file doesn’t work if the format of the data entry isn’t as specified. Also, the field length for this parameter is 10 characters. While the user inputs the value in this field, the first digit should align directly with the second letter of YMODU.
c. …show more content…
POSRA: This parameter specifies the Poisson's ratio of the substrate used. The value of POSRA for polyacrylamide gel of any stiffness should be entered as 3.000E-01.
d. TFILM: This parameter specifies the thickness of the substrate used. The units for this parameter are meters. One of the methods to record the thickness of the substrate used is to image the substrate embedded with fluorescent beads throughout the gel in confocal microscopy is to record the distance between the top and bottom layer of the fluorescent bead-PAA gel
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