Catalase Lab

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The purpose of this experiment is to examine the effect of Concentration of Catalase enzyme (E or E/2) and Concentration of Hydrogen Peroxide on the Mean Reaction Rate (ppm O2 evolved/min) and estimate the affinity of Catalase enzyme for Hydrogen Peroxide using Michaelis-Menton enzyme kinetics model by determining the Km and Vmax.
The results indicated that Catalase Enzyme Concentration and Concentration of Hydrogen Peroxide do affect the Mean Reaction Rate (ppm O2 evolved/min). The Figure 1 showed that Catalase enzyme E (Full Strength Enzyme Extract) graph has higher Mean Reaction Rate compared to Catalase enzyme E/2 (Half-Strength Enzyme Extract) graph. This is because, there were greater number of active sites accessible for the enzyme-substrate complex to be formed in the Catalase enzyme E compared to Catalase enzyme E/2 as the E/2 solution has been diluted with buffer and has one-half of the enzyme concentration. Catalase is very important because, it is an enzyme responsible for accelerating the lysis of Hydrogen Peroxide into water and Oxygen. If they were not present or had decreased in affinity for their substrate, …show more content…
As the amount of 3% Hydrogen Peroxide used was increased from 1ml to 2ml, 4ml and 5ml, the Mean Reaction Rate increased. When the concentration of Hydrogen Peroxide is doubled from 3% to 6%, the Mean Reaction Rate also went up. The Mean Reaction Rate for 5ml of 6% Hydrogen Peroxide was approximately 27293.9 ppm O2 evolved/min, which was higher compared to 5ml of 3% Hydrogen Peroxide that produced approximately 23126.5 ppm O2 evolved/min even though the same 5ml volume of substrate were used (Table 2, Figure 1). The increase in Mean Reaction Rate occurs because the more substrate, the more chance of collisions and effective collisions resulting in the more formation of enzyme-substrate

Catalase Lab

The purpose of this lab was to observe the effect adding catalase to a hydrogen peroxide solution. In the lab, titrations were preformed to determine the amount of hydrogen peroxide remaining after various times. The times used were 10, 30, 60, 120, 180, and 360 seconds. The hydrogen peroxide solution contained hydrogen peroxide, distilled water, and a catalase. After the reaction occurred the rate of reactions of the catalase over the specific period of time was calculated and graphed. In the…

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Potato Catalase Lab

energy of a substrate5. In this lab, hydrogen peroxide is added to six different samples of cells; raw hamburger, spinach leaf, raw liver, boiled liver, chalk and potato. The purpose of the lab is to observe the reaction of Hydrogen Peroxide with catalase in animal and plant cells, to compare the activity of catalase in animal cells and plant cells and to analyses the effects of boiling enzymes. Both animal and plant cells are known to contain catalase1-3,6. The lab will test to see which cells, plant…

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Yeast Vs Oxygen Lab

Temperature of Yeast vs Oxygen Lab Conclusion The temperature of yeast has an effect on the amount of oxygen released by catalase when breaking down Hydrogen Peroxide. In my Lab, my group and I tested for the amount of oxygen that was released by the catalase in yeast breaking down Hydrogen Peroxide with yeast at 3 different temperatures, 10ºC, 25ºC, 40ºC. We had 2 trials for each temperature tested. The average amount of oxygen released at 10ºC was 143 mL, the average for 25ºC was 123 mL and for…

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Enzyme Catalysis Lab TEACHER S GUIDE Essay

Catalase Enzyme Activity Enzyme Catalysis Lab Teacher’s Guide Table of Contents Teacher Guide Unit Overview Lesson Plan Materials and Preparation At least 1 day in Advance: Morning of Lab: Immediately Prior: Variations and Extensions Sample/Key Hindsight Student Guide Objective Standards Introduction Laboratory Sheet Safety Precautions Lab Team Materials Shared Class Materials Procedure Student Lab Sheet Catalase Enzyme Activity Created by Cheryl Fiello, Linda Moule, Marizka Rivette, Sarah Tonks…

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Enzyme Lab Essay examples

why we have enzyme, catalase (a protein), to break it down into two harmless element and compound. Catalase can be affect by the following factors: concentration of substrate, temperature, pH, salt concentration, and the presence of inhibitors. In the following equation, two molecules of hydrogen peroxide reacts with catalase to produce two water molecules and one molecule of oxygen gas: 2H2O2 → 2H2O +O2 In this lab, we are testing whether our hypothesis is correct: If catalase has a pH higher than…

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biology essay

Rico Duncan Microbiology Lab Thursday 12:30 Lab report antibiotic Introduction ` The first antibiotic, penicillin was discover in 1929 by sir alexander fleming. He made the discovery will observing a staphylococci agar that was contained by penicillin mold. Sir Alexander flemming discover that the mold had a space or an area where the staphylococcus didn’t grow. That single observation lead to a multiple array of antibiotics that we use today. Since this discovery, we have found…

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Biology Enzyme Lab Essay

Enzyme Lab Introduction/ Abstract An enzyme is a substance produced by a living organism that acts as a catalyst to bring about a specific biochemical reaction. They are mainly made up of proteins and can tremendously speed up reactions. E. coli ( a bacterium) has about 1,000 different types of enzymes floating around in its cytoplasm at any given time. Enzymes can be used to join and even break up molecules as shown in the diagram below. (1)…

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Enzyme and Enzyme Activity Background Essay

for Measuring Catalase (also called Peroxidase) Peroxide (such as hydrogen peroxide) is a toxic byproduct of aerobic metabolism. Catalase is an enzyme that breaks down these peroxides. It is produced by most cells in their peroxisomes. The general reaction can be depicted as follows: Enzyme + Substrate --> Enzyme-Substrate Complex --> Enzyme + Product(s) + ΔG For this investigation the specific reaction is as follows: Catalase + Hydrogen Peroxide --> Complex --> Catalase + Water + Oxygen…

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Unknown Bacteria Lab Report

bacteria. During the catalase test, the immediate formation of bubbles indicates that the bacteria contains high levels of catalase. The bacteria did grow on the mannitol salt agar plate, indicating that the bacteria is resistant to sodium chloride. The agar also appeared red meaning that the bacteria was unable to ferment the mannitol. With these results, it is possible to make a guess of what kind of bacteria the unknown was. With the bacteria being gram positive and catalase positive it suggests…

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Develop An Understanding Of The Metric System

Lab Report 1 Purpose To develop an understanding of the metric system and use it to measure common objects. To also learn about how to report uncertainty in your measurements. Lab Observations Most flat objects do not consist of uncertainty. When we measured round objects, because of its shape, it contained uncertainty. Lab Answers 1. Using a metric ruler, determine the length of the items in Table 1.1 below: In the final column, you are to estimate your measurement precision. To do…

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