Catalase Lab

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The purpose of this lab was to observe the effect adding catalase to a hydrogen peroxide solution. In the lab, titrations were preformed to determine the amount of hydrogen peroxide remaining after various times. The times used were 10, 30, 60, 120, 180, and 360 seconds. The hydrogen peroxide solution contained hydrogen peroxide, distilled water, and a catalase. After the reaction occurred the rate of reactions of the catalase over the specific period of time was calculated and graphed. In the experiment, the independent variable was the time, the dependent variable was the amount of hydrogen peroxide consumed, and a baseline assay was used as a control. A task that proteins have to is act as an enzyme, which are catalyst that increase …show more content…
Read the initial volume of KMnO4 in the syringe and record this in Data Table 3.
11. Holding the syringe over the titration vessel, carefully drop one drop of KMnO4 into the cup. Swirl the cup gently and observe the solution for any pink to brown discoloration that remains after mixing is complete.
12. Complete the titration by repeating Step 12, drop by drop, until a pink to brown color appears after mixing.
13. Record the final volume of the KMnO4 solution in the 12 mL syringe in Data Table 1.
14. Dispose of the sample in the titration vessel according to instructions from your teacher. Rinse and dry the titration vessel. Repeat the titration on 10 mL of each of the remaining reaction mixtures (10,30,60,120,180, 360 seconds). Be sure to clean and dry the titration vessel in between each titration. Record your data for each mixture in Data Table 4.
15. Wash your hands and clean up and dispose of any waste materials as directed by your teacher.
16. Perform the calculations for H2O2 decomposition and catalase reaction rates in Data
Table 3. The reaction rate data from Data Table 1 will then be used to construct a …show more content…
Sulfuric acid (H2SO4) is a strong acid and can be a caustic irritant if allowed to come in contact with the skin. In case of spills or skin contact, inform your teacher immediately and flush areas with running water for 15 minutes. Potassium permanganate (KMnO4) will stain skin, equipment, and clothing if contact occurs. Be sure to wear appropriate laboratory protective clothing and observe proper laboratory techniques to avoid contact with KMnO4. Dispose of any waste materials and clean up your work area as directed by your teacher. Be sure to always wash your hands before leaving the
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