Enzyme Lab Essay examples

Submitted By grewalharry96

Words: 320

Pages: 2

Open Document

TITLE: Investigating the reaction of hydrogen peroxide by the help of an enzyme
INTRODUCTION:
Hydrogen peroxide is weak acid with a pH of 6.2 but a highly toxic compound with the chemical formula of H2O2. The compound itself could be harmful if touched or swallowed and this is why we have enzyme, catalase (a protein), to break it down into two harmless element and compound. Catalase can be affect by the following factors: concentration of substrate, temperature, pH, salt concentration, and the presence of inhibitors. In the following equation, two molecules of hydrogen peroxide reacts with catalase to produce two water molecules and one molecule of oxygen gas:
2H2O2 → 2H2O +O2
In this lab, we are testing whether our hypothesis is correct:
If catalase has a pH higher than 7, then hydrogen peroxide would not be broken down.
BACKGROUND INFORMATION:
Hydrogen peroxide is used in the following:

Treatments: Tools:
Acne - Toothbrush
Mouthwash -Humidifiers / Steamers
Hair Lightening -Stain Remover
Teeth whitening, Tooth Ache -Laundry
Contact Lenses -Meat Sanitizing -Marinade -Bleachers -Disinfectants

MATERIALS NEEDED:
Hydrogen Peroxide 2 Blue litmus Paper Beaker
Catalase Erlenmeyer Flask Bleach
2 Graduated Cylinders Safety Goggles Toothpaste

PROCEDURE:
1. First, place a blue litmus paper into hydrogen peroxide to test whether the compound is acidic or basic
2. Pour hydrogen peroxide into the graduated cylinder till it reaches 25 ml
3. Pour the 25 ml H2O2 into the Erlenmeyer

Enzyme Lab

Introduction: Enzymes are a type of protein that initiate and assist every chemical reaction that occurs. Enzymes have the ability to do this with help from a few things. Increasing the temperature helps increase reaction rates however if you go past the optimum temperature it denaturizes the enzyme and the reaction rate decreases. PH can also have an effect on enzymes go past the optimum pH and it can disrupt the enzyme function in the interactions between excess hydrogen or hydroxide ions and…

Words 1417 - Pages 6

Enzyme Lab Report

of reactivity of an enzyme, and what the optimal pH for the enzyme is. The answer to these questions are yes, pH affects the reactivity rate of an enzyme, and the optimal pH for the enzyme is about 9. Answering the questions, pH greatly affects the rate of reactivity of an enzyme. This is evident in figure 2 above. Looking at the trend of the data, when the pH was either too basic, or too acidic, the rate of reactivity was very low. This happens because of denaturation. Enzymes have an optimal pH…

Words 621 - Pages 3

Lab Enzyme Essay

Observation: Enzymes are proteins that help speed up chemical reactions and as such, are subject to change when heat, salt or pH are involved. Enzymes have evolved to withstand such factors in which they reside so, if there are different factors like pH and heat involved, the effect of a specific enzyme may not work as well or even better in different conditions compared to other types of enzymes. Another type of factor as well is what kind of organism uses the enzyme and if they have any conditions…

Words 837 - Pages 4

Potato Enzyme Lab

A Study on Enzyme Kinetics by Substrate Concentration and pH Colin Kuehnel Abstract An enzyme is capable of increasing rate (speed) of a reaction by lowering the activation energy of said reaction, specifically in our case the enzyme catalase. Like most things in the world, enzymes are subject to their environments and have ideal conditions in which they perform the best. Our experiment tested the performance of catalase under a variety of conditions including fluctuating pH, presence of inhibitors…

Words 1890 - Pages 8

Enzyme Lab Report

Enzyme Lab Report Enzymes are proteins that help speed up chemical reactions in your body. They help with our digestion and our metabolism. When enzymes speed up our chemical reactions its called catalysis. In order for the enzymes to catalysis it needs help from a molecule called a substrate. The substrate then binds to an active site. Every active site has it’s own little molecule or molecule’s that fit it. Lactase is an enzyme that is found in the small intestine. It breaks down lactose (milk…

Words 1567 - Pages 7

Enzyme Lab Report

Enzyme Action: Testing Catalase Activity Introduction Many organisms can decompose hydrogen peroxide enzymatically. Enzymes are proteins that are responsible for most of the chemical activities of living organisms. Enzymes act as catalysts, or substances that speed up the rate of chemical reactions. Enzyme activity is affected greatly under various conditions, such as different enzyme concentrations, pH values, and temperatures. Purpose To determine if differences in enzyme concentration, pH values…

Words 1986 - Pages 8

Enzyme Lab Report

By completing the enzyme lab it became evident that temperature directly correlates with enzyme activity. The chilled water and hydrogen peroxide created the least amount of enzyme activity, the control the second most, and lastly the hot water and hydrogen peroxide had the most activity. The class data proves this by showing that when catalase and hot hydrogen peroxide were placed in hot water, 62 millimeters of oxygen were produced. The cold water and hydrogen peroxide only produced 28 millimeters…

Words 408 - Pages 2

Enzyme Lab Essay

LABORATORY REPORT (Click on the Save a Copy button on the panel above to save your report) Activity: Enzyme Activity Predictions 1. Sucrase will have the greatest activity at pH 6 2. Sucrase will have the greatest activity at 60 °C (140 °F) 3. Sucrase activity decreases with increasing sucrose concentration. Materials and Methods Effect of pH on Enzyme Activity. 1. Dependent Variable. amount of product (glucose and fructose) produced 2. Independent Variable. pH 3. Controlled Variables…

Words 1196 - Pages 5

Enzyme Lab Report

accelerate chemical reactions is known as an enzyme. Enzymes contain a specific site where the reaction will take place, known as the active site. This site is unique where only a certain type of molecule, substrate, may attach. The reaction will take place and the products of the reaction will be released. There are two main hypotheses on the shape of an enzyme. The first one is the lock and key hypothesis. This hypothesis explains that the shape of the enzyme and the shape of the substrate are so specific…

Words 319 - Pages 2

Lactase Enzyme Lab Report

Lactase Enzyme Lab This lab will examine the specificity of an enzyme (lactase) to a specific substrate (lactose). You and your lab partner will observe the actions of the enzyme and how shape is important to enzyme reactions. Lactose, the sugar found in milk, is a disaccharide composed of glucose and galactose (both six-sided sugars). Sucrose, ordinary table sugar, is also a disaccharide composed of fructose and glucose. Glucose is a six-sided sugar and fructose is a five-sided sugar. Lactase…

Words 569 - Pages 3